Hemin competitively inhibits HSPA8 ATPase activity mitigating its foldase function.

Hemolysis in red blood cells followed by hemoglobin degradation results in high hemin levels in the systemic circulation. Such a level of hemin is disastrous for cells and tissues and is considerably responsible for the pathologies of diseases like severe malaria. Hemin's hydrophobic chemical nature and structure allow it to bind several proteins leading to their functional modification. Such modifications in physiologically relevant proteins can have a high impact on various cellular processes. HSPA8 is a chaperone that has a protective role in oxidative stress by aiding protein refolding. Through ATPase activity assays we found that hemin can competitively inhibit ATP hydrolysis by the chaperone HSPA8. Hemin as such does not affect the structural integrity of the protein which is inferred from CD spectroscopy and Gel filtration but it hinders the ATP-dependent foldase function of the chaperone. HSPA8 was not able to cause the refolding of the model protein lysozyme in the presence of hemin. The loss in HSPA8 function was due to competition between hemin and ATP as the chaperone was able to regain the foldase function when the concentration of ATP was gradually increased with hemin present at the inhibitory concentration. In-silico studies to establish the competition for the specific binding site revealed that ATP was unable to replace hemin from the ATP binding pocket of HSPA8 and was forced to form a non-specific and unstable complex. In-vitro isothermal calorimetry revealed that the affinity of ATP for binding to HSPA8 was reduced 22 folds in the presence of hemin. The prevention of HSPA8's cytoprotective function by hemin can be a major factor contributing to the overall cellular damage during hemin accumulation in the case of severe malaria and other hemolytic diseases.

Efficient Surface Passivation of Ti-Based Layered Materials by a Nonfluorine Branched Copolymer for Durable and High-Power Sodium-Ion Batteries.

There is a crucial need for low-cost energy storage technology based on abundant sodium ions to realize sustainable development with renewable energy resources. Poly(vinylidene fluoride) (PVDF) is applied as a binder in sodium-ion batteries (SIBs). Nevertheless, PVDF is also known to suffer from a larger irreversible capacity, especially when PVDF is used as the binder of negative electrode materials. In this research, a poly(acrylonitrile)-grafted poly(vinyl alcohol) copolymer (PVA-g-PAN) is tested as a binder with Ti-based layered oxides as potential negative electrode materials for SIBs. The chemical stability tests of PVDF and PVA-g-PAN contacted with metallic sodium have been conducted, which reveals that PVDF experiences a defluorination process, while PVA-g-PAN demonstrates excellent chemical stability. Composite electrodes with PVA-g-PAN demonstrate superior electrochemical performances when compared with the PVDF binder, allowing improvement for initial CE, higher rate capability, and long cyclability over 1500 cycles. Detailed characterization of electrodes via soft X-ray photoelectron spectroscopy and field emission scanning electron microscopy demonstrates that the PVA-g-PAN branched structure allows a more uniform distribution of acetylene black with higher coatability, unlocking enhanced rate performances and efficient passivation of Ti-based oxides without the excessive electrolyte decomposition. These findings open a new way to design practical and durable sodium-ion batteries with a high-power density.

In Vitro Effects of Zirconia Nanoparticles: Uptake, Genotoxicity, and Mutagenicity in V-79 cells.

Zirconia nanoparticles are used in various industrial and biomedical applications such as dental implants, thermal barrier sprays, and fuel cells. The interaction of nanoparticles with the environment and humans is inevitable. Despite the enormous application potential of these nanoparticles, there are still some gaps in the literature regarding potential toxicological mechanisms and the genotoxicity of zirconia nanoparticles. The lung is one of the main exposure routes to nanomaterials; therefore, the present study was designed to determine the genotoxic and mutagenic effect of zirconia NPs in V-79 lung cells. Zirconia nanoparticles showed significant internalization in cells at 100 µg/mL and 150 µg/mL concentrations. Zirconia nanoparticles showed low cytotoxicity and were found to generate ROS in V-79 cells. In alkaline comet assay, zirconia nanoparticles (10 µg/mL, 50 µg/mL, and 100 µg/mL) exposed cells exhibited significant DNA strand breaks, while the neutral comet assay, which was used for double-strand break assessment, only revealed significant damage at 100 µg/mL. Chromosomal aberration induced by zirconia nanoparticles mainly resulted in the generation of gaps, few fragments, and breaks which signifies the low clastogenic activity of these nanoparticles in the V-79 cell line. In MN assay, zirconia nanoparticles resulted in no significant micronuclei induction at any given concentration. In the HPRT mutation assay, the particle shows a dose-dependent increase in the mutant frequency. It is evident from the result that zirconia nanoparticles cause dose-dependent cytotoxicity and genotoxicity, but still, more studies are needed to evaluate the clastogenic potential and the possible mechanism involved.

Role Transformation of HSPA8 to Heme-peroxidase After Binding Hemin to Catalyze Heme Polymerization.

Hemin, a byproduct of hemoglobin degradation, inflicts oxidative insult to cells. Following its accumulation, several proteins are recruited for heme detoxification with heme oxygenase playing the key role. Chaperones play a protective role primarily by preventing protein degradation and unfolding. They also are known to have miscellaneous secondary roles during similar situations. To discover a secondary role of chaperones during heme stress we studied the role of the chaperone HSPA8 in the detoxification of hemin. In-silico studies indicated that HSPA8 has a well-defined biophoric environment to bind hemin. Through optical difference spectroscopy, we found that HSPA8 binds hemin through its N-terminal domain with a Kd value of 5.9 ± 0.04 µM and transforms into a hemoprotein. The hemoprotein was tested for exhibiting peroxidase activity using guaiacol as substrate. The complex formed reacts with H2O2 and exhibits classical peroxidase activity with an ability to oxidize aromatic and halide substrates. HSPA8 is dose-dependently catalyzing heme polymerization through its N-terminal domain. The IR results reveal that the polymer formed exhibits structural similarities to ß-hematin suggesting its covalent nature. The polymerization mechanism was tested through optical spectroscopy, spin-trap, and activity inhibition experiments. The results suggest that the polymerization occurs through a peroxidase-H2O2 system involving a one-electron transfer mechanism, and the formation of free radical and radical-radical interaction. It highlights a possible role of the HSPA8-hemin complex in exhibiting cytoprotective function during pathological conditions like malaria, sickle cell disease, etc.

Forecasting global plastic production and microplastic emission using advanced optimised discrete grey model.

Plastic pollution has become a prominent and pressing environmental concern within the realm of pollution. In recent times, microplastics have entered our ecosystem, especially in freshwater. In the contemporary global landscape, there exists a mounting apprehension surrounding the manifold environmental and public health issues that have emerged as a result of the substantial accumulation of microplastics. The objective of the current study is to employ an enhanced grey prediction model in order to forecast global plastic production and microplastic emissions. This study compared the accuracy level of the four grey prediction models, namely, EGM (1,1, α, θ), DGM (1,1), EGM (1,1), and DGM (1,1, α) models, to evaluate the accuracy levels. As per the estimation of the study, DGM (1,1, α) was found to be more suitable with higher accuracy levels to predict microplastic emission. The EGM (1,1, α, θ) model has slightly better accuracy than the DGM (1,1, α) model in predicting global plastic production. Various accuracy measurement tools (MAPE and RMSE) were used to determine the model's efficiency. There has been a gradual growth in both plastic production and microplastic emission. The current study using the DGM (1,1, α) model predicted that microplastic emission would be 1,084,018 by 2030. The present study aims to provide valuable insights for policymakers in formulating effective strategies to address the complex issues arising from the release of microplastics into the environment and the continuous production of plastic materials.

Temperature-induced lipocalin-mediated membrane integrity: Possible implications for vindoline accumulation in Catharanthus roseus leaves.

Plant lipocalins perform diverse functions. Recently, allene oxide cyclase, a lipocalin family member, has been shown to co-express with vindoline pathway genes in Catharanthus roseus under various biotic/abiotic stresses. This brought focus to another family member, a temperature-induced lipocalin (CrTIL), which was selected for full-length cloning, tissue-specific expression profiling, in silico characterization, and upstream genomic region analysis for cis-regulatory elements. Stress-mediated variations in CrTIL expression were reflected as disturbances in cell membrane integrity, assayed through measurement of electrolyte leakage and lipid peroxidation product, MDA, which implicated the role of CrTIL in maintaining cell membrane integrity. For ascertaining the function of CrTIL in maintaining membrane stability and elucidating the relationship between CrTIL expression and vindoline content, if any, a direct approach was adopted, whereby CrTIL was transiently silenced and overexpressed in C. roseus. CrTIL silencing and overexpression confirmed its role in the maintenance of membrane integrity and indicated an inverse relationship of its expression with vindoline content. GFP fusion-based subcellular localization indicated membrane localization of CrTIL, which was in agreement with its role in maintaining membrane integrity. Altogether, the role of CrTIL in maintaining membrane structure has possible implications for the intracellular sequestration, storage, and viability of vindoline.

Conservation of orbital angular momentum throughout amplification of high order harmonics in Ni-like krypton and silver plasmas.

In this article we present modelling results of the amplification of High Order Harmonics (HOH) carrying orbital angular momentum (OAM) in plasma amplifiers created from krypton gas and silver solid targets. The resulting amplified beam is characterized in terms of intensity, phase and decomposition in helical and Laguerre-Gauss modes. Results show that the amplification process conserves OAM, although some degradation is apparent. Several structures appear in the intensity and phase profiles. These structures have been characterized with our model and related to refraction and interference with the plasma self-emission. Thus, these results not only demonstrate the capability of plasma amplifiers to deliver HOH amplified beams carrying OAM but also pave the way towards using HOH carrying OAM as a probe beam to diagnose the dynamics of hot, dense plasmas.

Tribal Malaria in India: An Analysis of Malaria Incidence and Mortality over 20-Year Period (2000-2020).

Objective: Tribal malaria is well known for its substantial share in the overall malarial load of the country. This paper examines the levels and trends of malaria incidence and mortality in the tribal population for the past two decades. Methods: Data on malaria incidence and mortality were collected from an online e-repository that provides statistical data and information on 19 sectors, including health. Results: The analysis showed that the malaria incidence and mortality in tribal-dominated regions declined at an average annual rate of 4.3% per annum between 2000 and 2020, which accompanies the tremendous progress made in malaria control at the country level during this time period. The results also showed that between 2016 and 2020, the decline in tribal-dominated regions was consistent and noteworthy in terms of magnitude, a period that marks the implementation of the national framework for malaria elimination in the country. Conclusion: The decisive fall in the incidence and mortality of malaria in the tribal-dominated region has put India on track to achieve the target of 3.3 of the Sustainable Development Goals. However, with the pandemic impacting service delivery, monitoring, and reporting, including malaria control programs, it is important to maintain the momentum of progress in malaria control.

To compare the outcomes of pancreaticojejunostomy and pancreaticogastrostomy reconstruction after pancreaticoduodenectomy: A prospective observational study.

Background: We have been in constant search of novel innovations to decrease the high morbidity after Pancreaticoduodenectomy (PD). Pancreaticojejunostomy (PJ) and pancreaticogastrostomy (PG) are the two different methods of reconstruction after PD. However, the existing data is ambiguous in supporting either of them as the preferred technique of reconstruction. Methods: This was a single-center prospective observational study that included 64 patients who underwent PD over two years. We compared PG with PJ as a method of reconstruction after PD. The primary objective was to assess whether PG decreases the rate of postoperative pancreatic fistula (POPF) rates or not. Secondary objectives comprised analysis of perioperative outcomes, 30-day and 90-day mortality. Results: Pancreatic fistula was significantly lower in PG as compared to the PJ group (24% vs. 47%) with a p-value of 0.027. The incidence of clinically pertinent (grade B) fistula was only 3% in the PG group and 32% in the PJ group. PG group had a higher incidence of post pancreatectomy hemorrhage (PPH) and delayed gastric emptying (DGE). No statistically significant difference was seen between either group need for blood transfusion, re-exploration, re-admissions, ICU stay, or length of hospital stay, and 30-day and 90-day mortality. Pancreatic texture and high BMI were independent predictors for pancreatic fistula. Conclusion: PG when compared to PJ for reconstruction after PD, decreases the rate of POPF significantly; however, it is associated with an elevated risk of DGE and PPH. There was no difference in 30-day and 90-day mortality between both the treatment groups.

Prediction of surface temperature and CO2 emission of leading emitters using grey model EGM (1,1, α, θ).

The current study projects the increase in surface temperature and CO2 emissions using the EGM (1,1, α, θ) grey model for the six most significant CO2 contributing countries, namely China, the USA, India, Russia, Japan, and Germany. The study uses time series data for surface temperature (in degree celsius) from 2010 to 2020, and CO2 emission (metric tons per capita) data from 2009 to 2019. The empirical results show a downward trend in CO2 emissions from Japan, Germany, the USA, and Russia by 2028. However, in the same time period, CO2 emissions are expected to increase for India and remain nearly constant for China. This study indicates an increase in surface temperature at a significant rate in all the 6 countries: by 6.70 °C for China, 7.52 °C for Germany, 2.95 °C for India, 2.66 °C for Japan, 3.61 °C for Russia, and 13.48 °C for the USA by the end of 2028. The study compares the EGM (1,1, α, θ) grey model with the general EGM (1,1) grey model and finds that the EGM (1,1, α, θ) model performs better in both in-sample and out-of-sample forecasting. The paper also puts forward policy suggestions to mitigate, manage, and reduce increases in surface temperature as well as CO2 emissions.

Evaluating chemicals of emerging concern in the Ganga River at the two major cities Prayagraj and Varanasi through validated analytical approaches.

Evaluating environmental water quality means to assess and protect the environment against unfriendly impacts from various organic impurities emerging from industrial emissions and those released during harvesting. Potential risks related with release of polycyclic aromatic hydrocarbons (PAHs), pesticides and pharmaceuticals (PhAcs), and personal care products (PCPs) into the environment have turned into an increasingly serious issue in ecological safety. Monitoring helps in control of chemicals and ecological status compliance to safeguard specific water uses, for example, drinking water abstraction. A longitudinal review was carried out for 55 different persistent organic pollutants (POPs) for the Ganga River which passes through the urban areas of Prayagraj and Varanasi, India, through validated analytical approaches and measurement uncertainty (MU) estimation to assess their potential use for routine analysis. Furthermore, environmental risk assessment (ERA) carried out in the present study has revealed risk quotient (RQ) higher than 1 in a portion of the aquatic bodies. Using a conservative RQ strategy, POPs were assessed for having extensive risks under acute and chronic exposure, proposing that there is currently critical ecological risk identified with these compounds present in the Ganga River. In general, these outcomes demonstrate a significant contribution for focusing on measures and feasible techniques to minimize the unfavorable effects of contaminants on the aquatic environment.

Forecasting of non-renewable and renewable energy production in India using optimized discrete grey model.

Renewable energy delivers reliable power supplies and fuel diversification, enhancing energy security and lowering fuel spill risk. Renewable energy also helps conserve the nation's natural resources. Solar and other renewable energy sources have become increasingly prominent in recent years. India has achieved the 20 GW capacity solar energy production target before 2022. It is presently producing the lowest-cost solar power at the global level. Thermal energy has dominated the energy market. Countries have decided on energy generation from renewable sources and adopting green energy. This study forecasted non-renewable and renewable energy from multiple sources (hydropower, solar, wind and bioenergy) using grey forecasting model DGM (1,1,α). The comparative analyses with the classical models DGM (1,1) and EGM (1,1) revealed the superiority of the DGM (1,1,α). We also used CAGR for 2009-2019 to compare the actual and predicted data growth rate. The results show that non-renewable and renewable energy production is expected to increase. However, renewable energy generation wind sources continue to increase faster than hydropower, solar and bioenergy.

Ultrastructural Changes in Autopsy Tissues of COVID-19 Patients.

INTRODUCTION: The COVID-19 pandemic resulted in substantial morbidity and mortality across the world. The prognosis was found to be poor in patients with co-morbidities such as diabetes, hypertension, interstitial lung disease, etc. Although biochemical studies were done in patient samples, no study has been reported from the Indian subcontinent about ultrastructural changes in the vital organs of COVID-19 patients. The present study was, therefore, conducted to understand the ultrastructural changes in the lung, liver, and brain of the deceased patients. METHODS: The present study was conducted on samples obtained from reverse transcription-polymerase chain reaction (RT-PCR)-positive patients who were admitted to a tertiary care hospital in Western India. Core needle biopsies were done in eight fatal cases of COVID-19. The samples were taken from the lungs, liver, and brain and subjected to light microscopy, immunohistochemistry (IHC), and transmission electron microscopy (TEM). Clinical details and biochemical findings were also collected.  Results: The study participants included seven males and one female. The presenting complaints included fever, breathlessness, and cough. Light microscopy revealed diffuse alveolar damage in the lungs. Further, a positive expression of SARS-CoV-2 nucleocapsid protein was observed in the pulmonary parenchyma of five patients. Also, the TEM microphotograph showed viral particles of size up to 80nm localized in alveolar epithelial cells. However, no viral particles were found in liver or brain samples. In the liver, macrovesicular steatosis and centrizonal congestion with loss of hepatocytes were observed in light microscopy. CONCLUSION:  This is the first study in the Indian population showing the in-situ presence of viral particles in core biopsies from fatal cases of COVID-19. As evident from the results, histology and ultrastructural changes in the lung correlated with the presence of viral particles. The study revealed a positive correlation between the damage in the lungs and the presence of viral particles.

Physicochemical characterization and oxidative potential of size fractionated Particulate Matter: Uptake, genotoxicity and mutagenicity in V-79 cells.

For many years, the impact of Particulate Matter (PM) in the ambient air has been one of the major concerns for the environment and human health. The consideration of the heterogeneity and complexity of different size fractions is notably important for the assessment of PM toxicological effects. The aim of the study was to present a comprehensive size-composition-morphology characterization and to assess the oxidative potential, genotoxicity, and mutagenicity of the atmospheric PM fractions, collected by using MOUDI near a busy roadside in Lucknow, India. Physicochemical characterization of ambient coarse particles (1.8-10 µm), fine particles (0.32-1.8 µm), quasi-ultrafine (0.1-0.32 µm) and ultrafine particles (≤0.1 µm) along with SRM 1649b was done using TEM, SEM, DLS, NTA, ICP-MS, and IC in parallel with the estimation of exogenous Reactive Oxygen Species (ROS) by acellular assays. In this study, two different acellular assays, dithiothreitol (DTT) and the CM-H2DCFDA assay, indicated stronger mass-normalized bioactivity for different size ranges. Enrichment factor analysis indicated that the different size fractions were highly enriched with elements of anthropogenic origin as compared to elements of crustal origin. The endotoxin concentration in different size fractions was also estimated. Cellular studies demonstrated significant uptake, cytotoxicity, ultrastructural changes, cellular ROS generation, and changes in the different phases of the cell cycle (Sub G1, G1, S, G2/M) exposed to different size fractions. The Comet assay and the Micronucleus assay were used to estimate genotoxicity. Mutagenic potential was revealed by the HGPRT gene forward mutation assay in V-97 cells. Conclusively, our results clearly indicate that the genotoxic and mutagenic potential of the coarse PM was greater than the other fractions, and interestingly, the ultrafine PM has higher bioactivity as compared to quasi-ultrafine PM.

Ocimum sanctum, OscWRKY1, regulates phenylpropanoid pathway genes and promotes resistance to pathogen infection in Arabidopsis.

KEY MESSAGE: OscWRKY1 from Ocimum sanctum positively regulates phenylpropanoid pathway genes and rosmarinic acid content. OscWRKY1 overexpression promotes resistance against bacterial pathogen in Arabidopsis. WRKY transcription factor (TF) family regulates various developmental and physiological functions in plants. PAL genes encode enzymes which are involved in plant defense responses, but the direct regulation of PAL genes and phenylpropanoid pathway through WRKY TF's is not well characterized. In the present study, we have characterized an OscWRKY1 gene from Ocimum sanctum which shows induced expression by methyl jasmonate (MeJA), salicylic acid (SA), and wounding. The recombinant OscWRKY1 protein binds to the DIG-labeled (Digoxigenin) W-box cis-element TTGAC[C/T] and activates the LacZ reporter gene in yeast. Overexpression of OscWRKY1 enhances Arabidopsis resistance towards Pseudomonas syringae pv. tomato Pst DC3000. Upstream activator sequences of PAL and C4H have been identified to contain the conserved W-box cis-element (TTGACC) in both O. sanctum and Arabidopsis. OscWRKY1 was found to interact with W-box cis-element present in the PAL and C4H promoters. Silencing of OscWRKY1 using VIGS resulted in reduced expression of PAL, C4H, COMT, F5H and 4CL transcripts. OscWRKY1 silenced plants exhibit reduced PAL activity, whereas, the overexpression lines of OscWRKY1 in Arabidopsis exhibit increased PAL activity. Furthermore, the metabolite analysis of OscWRKY1 silenced plants showed reduced rosmarinic acid content. These results revealed that OscWRKY1 positively regulates the phenylpropanoid pathway genes leading to the alteration of rosmarinic acid content and enhances the resistance against bacterial pathogen in Arabidopsis.

Double hierarchy hesitant fuzzy linguistic information based framework for personalized ranking of sustainable suppliers.

With the growing appetite for reducing carbon footprint, organizations are tirelessly working towards green practices and one such crucial practice is purchasing raw materials from sustainable suppliers (SSs). Inspired by the drift in purchase habits, several sustainable suppliers emerged in the market and a rational selection of a suitable sustainable supplier is a complex decision problem. There are many criteria associated with the evaluation of sustainable suppliers, and double hierarchy hesitant fuzzy linguistic (DHHFL) structure is a popular preference style that accepts complex linguistic expressions in the natural language form. Earlier studies on sustainable supplier selection infer that (i) complex linguistic expressions are not properly modeled, (ii) interrelationship among criteria must be considered during importance assessment, (iii) direct assignment of attitudinal values of experts causes bias and subjectivity, and (iv) nature of criteria play a crucial role in ranking SSs. To overcome these limitations, a novel MCMD framework is proposed in this study in which the attitudinal characteristic values of experts are calculated by using a variance approach. Besides, importance of diverse sustainable criteria is calculated by proposing novel attitude-CRITIC approach that supports proper capturing of interrelationship among criteria along with experts' attitude values. Later, weighted distance approximation algorithm is presented to DHHFL setting for personalized and cumulative ranking of SSs by properly considering nature of criteria. These methods are integrated to form a framework under DHHFL setting, and its usefulness is exemplified by using a case study of SS selection in an automotive firm. A comprehensive sensitivity analysis as well performed to test the validity of the proposed model approves the applicability, validity, and robustness of the model. Lastly, comparison is done with other methods to understand the merits and shortcomings of the proposal.

Engineered Nanoparticle-Protein Interactions Influence Protein Structural Integrity and Biological Significance.

Engineered nanoparticles (ENPs) are artificially synthesized particles with unique physicochemical properties. ENPs are being extensively used in several consumer items, elevating the probability of ENP exposure to biological systems. ENPs interact with various biomolecules like lipids, proteins, nucleic acids, where proteins are most susceptible. The ENP-protein interactions are mostly studied for corona formation and its effect on the bio-reactivity of ENPs, however, an in-depth understanding of subsequent interactive effects on proteins, such as alterations in their structure, conformation, free energy, and folding is still required. The present review focuses on ENP-protein interactions and the subsequent effects on protein structure and function followed by the therapeutic potential of ENPs for protein misfolding diseases.

Cobalt oxide (Co3O4) nanoparticles induced genotoxicity in Chinese hamster lung fibroblast (V79) cells through modulation of reactive oxygen species.

Incessant production, pervasive applications in different fields, and eventually unintended exposure of cobalt oxide nanoparticles (Co3O4 NPs) lead to rise in their toxicity studies toward human health. However, the information regarding the potential toxicity mechanisms of Co3O4 NPs especially genotoxicity is still sparse with missing interconnections. So far, only solitary reports on Co3O4 NPs are at hand, bearing witness to reactive oxygen species (ROS)-mediated DNA damage in lung cells. To address this, we evaluated the Co3O4 NP-induced cytotoxic and genotoxic potential in Chinese hamster lung fibroblast cell line (V79). Our preliminary results demonstrate that Co3O4 NPs at concentrations of 20-100 µg/ml induced moderate mortality after 24-h exposure. However, these low concentrations caused a significant reduction in various organelles' activity in a concentration-dependent manner. Mitochondrial activity and membrane potential were found to be compromised due to NP exposure in a concentration-dependent manner. The study affirms that Co3O4 NPs inhibited lysosomal activity in V79 cells. In addition to this, Co3O4 NPs are also found to stimulate free oxygen radical generation. Genotoxicity studies revealed a potent and dose-dependent effect of non-cytotoxic concentrations of Co3O4 NPs in the induction of DNA lesions. Interestingly, N-acetylcysteine, a free oxygen radical scavenger (5, 10 mM, pretreatment) inhibited the progression of free oxygen radicals and induction of Co3O4 NP-mediated DNA lesions. This suggests the ROS-mediated genotoxic potential of Co3O4 NPs.

Multiple forecasting approach: a prediction of CO2 emission from the paddy crop in India.

This paper compares four prediction methods, namely random forest regressor (RFR), SARIMAX, Holt-Winters (H-W), and the support vector regression (SVR), to forecast the total CO2 emission from the paddy crop in India. The major objective of this study is to compare these four models and suggest an effective model for the prediction of total CO2 emission. Data from 1961 to 2018 has been categorised into two parts: training and test data. The study forecasts total CO2 emission from paddy crops in India from 2019 to 2025. A comparison of mean absolute percentage error (MAPE) and the mean square error (MSE) highlights the differences in accuracy among the four models. The mean absolute percentage eror (MAPE) and the mean square error (MSE) for the four methods are RFR (MAPE: 5.67; MSE: 549,900.02), SARIMAX (MAPE: 1.67; MSE:70,422.35), H-W (MAPE:0.75; MSE:16,648.58), and SVR (MAPE: 0.91; MSE: 17,832.4). The values of MAPE and MSE with the Holt-Winters (H-W) and the support vector regression (SVR) are relatively low as compared to SARIMAX and RFR. Based on these results, it can be inferred that H-W and SVR were found suitable models to forecast the total CO2 emission from paddy crops. Holt-Winters model predicted 14,364.97 for the year 2025, and SVR predicted 13,696.67 for the year 2025. The decision-maker can use these predictions to build a suitable policy for the future. This approach can be contrasted with other forecasting methods, such as the neural network, and train the model to achieve better forecast accuracy.

Plasmodium falciparum Cysteine-Rich Protective Antigen (CyRPA) Elicits Detectable Levels of Invasion-Inhibitory Antibodies during Natural Infection in Humans.

Plasmodium falciparum cysteine-rich protective antigen (CyRPA) is a conserved component of an essential erythrocyte invasion complex (RH5/Ripr/CyRPA) and a target of potent cross-strain parasite-neutralizing antibodies. While naturally acquired human RH5 antibodies have been functionally characterized, there are no similar reports on CyRPA. Thus, we analyzed the parasite-neutralizing activity of naturally acquired human CyRPA antibodies. In this regard, CyRPA human antibodies were measured and purified from malaria-infected plasma obtained from patients in central India and analyzed for their parasite neutralizing activity via in vitro growth inhibition assays (GIA). We report that, despite being susceptible to antibodies, CyRPA is a highly conserved antigen that does not appear to be under substantial immune selection pressure, as a very low acquisition rate for anti-CyRPA antibodies was reported in malaria-exposed Indians. We demonstrate for the first time that the small amounts of natural CyRPA antibodies exhibited functional parasite-neutralizing activity and that a CyRPA-based vaccine formulation induces highly potent antibodies in rabbits. Importantly, the vaccine-induced CyRPA antibodies exhibited a robust 50% inhibitory concentration (IC50) of 21.96 µg/ml, which is comparable to the IC50 of antibodies against the leading blood-stage vaccine candidate, reticulocyte-binding-like homologous protein 5 (RH5). Our data support CyRPA as a unique vaccine target that is highly susceptible to immune attack but is highly conserved compared to other leading candidates such as MSP-1 and AMA-1, further substantiating its promise as a leading blood-stage vaccine candidate.